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What is the most appropriate method for sterilizing a heat-labile liquid solution containing essential proteins for use in tissue culture?
A. Filtration through a 0.22 µm pore size membrane filter
B. Irradiation with ultraviolet (UV) light
C. Exposure to ethylene oxide gas
D. Autoclaving at 121¡ãC for 15 minutes
Answer: A
NEW QUESTION # 176
A Gram stain of anaerobic growth from a peritoneal fluid abscess reveals pleomorphic, pale-staining Gram- negative rods. The isolate grows on Bacteroides Bile Esculin (BBE) agar, hydrolyzing esculin (producing black colonies), and is resistant to kanamycin (1000 µg), vancomycin (5 µg), and colistin (10 µg) disks. Which organism group is most likely?
A. Bacteroides fragilis group
B. Fusobacterium nucleatum
C. Clostridium perfringens
D. Prevotella melaninogenica group
Answer: A
NEW QUESTION # 177
A clinical microbiology laboratory is preparing a Gram stain of a mixed bacterial culture containing both Gram-positive and Gram-negative organisms. The correct order of reagents used in the Gram staining procedure is:
A. Safranin, crystal violet, decolorizer, iodine
B. Decolorizer, crystal violet, iodine, safranin
C. Iodine, crystal violet, decolorizer, safranin
D. Crystal violet, iodine, decolorizer, safranin
Answer: D
NEW QUESTION # 178
A clinical microbiology laboratory encounters a Gram-negative bacillus isolated from a bloodstream infection. The isolate exhibits resistance to multiple antibiotics, including carbapenems. Further phenotypic testing reveals the production of a metallo-beta-lactamase (MBL). Which of the following antibiotic combinations might still retain activity against this isolate?
A. Aztreonam
B. Ceftazidime-avibactam
C. Aztreonam-avibactam
D. Piperacillin-tazobactam
Answer: A
NEW QUESTION # 179
During a primary infection with Epstein-Barr virus (EBV), the virus establishes latency in B lymphocytes.
The transition from lytic to latent infection is tightly regulated by viral gene expression. Which of the following viral proteins is crucial for the establishment and maintenance of EBV latency and is a key target in EBV-associated lymphomas?
A. Epstein-Barr nuclear antigen 1 (EBNA1)
B. Glycoprotein gp350
C. Early antigen (EA)
D. Viral capsid antigen (VCA)
Answer: A
NEW QUESTION # 180
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